human embryonic kidney hek 293t cell lines  (ATCC)

Journal: Alzheimer's & Dementia

Article Title: NRN1 as a therapeutic target for Alzheimer's disease

doi: 10.1002/alz.71149

Figure Lengend Snippet: Abcam ab64186 recognizes NRN1 protein in mouse neuroblastoma cells. (A) Representative Western blot of human embryonic kidney (HEK) 293T, Neuro‐2a (N2a) mouse neuroblastoma, and SH‐SY5Y human neuroblastoma cell lysates (50 µg protein), probed with NRN1 polyclonal antibody Abcam ab64186 and GAPDH monoclonal antibody. (B) N2a cells were transfected with NRN1 or Scramble (non‐targeting) siRNA smart pools and harvested after 96 h. Western blot analyses with NRN1 polyclonal antibody Abcam ab64186 revealed reduced intensity of the ∼34 kDa band in NRN1‐depleted cells, compared to Scramble control. 10 µg of protein was loaded per lane, and GAPDH was probed as a loading control. GAPDH, glyceraldehyde‐3‐phosphate dehydrogenase; NRN1, Neuritin‐1; siRNA, small interfering RNA;

Article Snippet: Human embryonic kidney (HEK) 293T cell lines (Catalog No.: CRL‐3216, ATCC) were used.

Techniques: Western Blot, Transfection, Control, Small Interfering RNA

Journal: Nature Communications

Article Title: Targeting TNK2/ACK1 reverses the immunosuppressive tumor microenvironment and synergizes with immunochemotherapy in pancreatic cancer

doi: 10.1038/s41467-025-67197-3

Figure Lengend Snippet: a The Venn diagram illustrating the strategy for the screening of the candidate genes encoding proteins phosphorylated by TNK2. b The co-immunoprecipitation assay coupled with western blotting showing the interaction between overexpressed TNK2 and STAT5a in HEK-293T cells. c Western blotting analysis of indicated protein expression in related KPC cell lines. d Western blotting analysis of indicated proteins in in vitro kinases assays for measuring the activity of wild type and mutant TNK2 using STAT5a as substrate. e Western blotting analysis of indicated proteins in related KPC cell lines. f A schematic diagram showing the potential binding sites of STAT5a on the mouse HVEM promoter. g , h ChIP-qPCR analysis of the binding of STAT5a to the 3 potential binding sites on the HVEM promoter in indicated KPC cell lines ( n = 3 independent experiments). i A schematic diagram showing the mutations in the binding sites of STAT5a on the mouse HVEM promoter. j The dual luciferase analysis of the reporters containing wild type and the STAT5a binding site mutants of the HVEM promoter co-transfected into HEK-293T cells with TNK2, STAT5a or both ( n = 3 independent experiments). Statistical significance was determined by two-tailed unpaired Student’s t test. Data represent as mean ± s.d. Source data are provided as a Source Data file.

Article Snippet: The human PDAC cell lines AsPC-1, MIA PaCa-2, Panc 10.05, Capan-2, CFPAC-1 BxPC-3 and SW1990, and human embryonic kidney cell line HEK-293T, were purchased from the American Type Culture Collection (ATCC, USA).

Techniques: Co-Immunoprecipitation Assay, Western Blot, Expressing, In Vitro, Activity Assay, Mutagenesis, Binding Assay, ChIP-qPCR, Luciferase, Transfection, Two Tailed Test